Journal: Molecular Oncology
Article Title: A single lysis solution for the analysis of tissue samples by different proteomic technologies
doi: 10.1016/j.molonc.2008.09.003
Figure Lengend Snippet: Compatibility of the CLB1 solution with the antibody protein microarray format. The tissue sample was frozen‐sectioned and solubilized in either Panorama® Antibody Microarray Extraction buffer or in CLB1 lysis solution and labeled with ...
Article Snippet: The resulting lysate solutions were then serially 2‐fold diluted three times with CLB1 buffer and transferred onto FAST nitrocellulose microarray slides (Schleicher & Schuell BioScience, Germany) in quintuplicates using a MicroCasterTM Hand‐Held Microarrayer System (Schleicher & Schuell BioScience, Germany) according to manufacturer's instructions.
Techniques: Microarray, Extraction, Lysis, Labeling